Materials & Methods List

Cloning	Isolating M13 Clones and Preparing Infected Cultures
Cloning	Transformation
Competent Cell	Rb-Mn Treated Competent Cells
DNA Analysis	DNase I footprinting
	Exo III footprinting
	TAB restriction buffer
DNA Cloning	Standard Ligation
DNA Concentration	EtBr spot test
DNA Isolation	Genomic DNA isolation
DNA Isolation	Sephadex G-50 Spin Column
DNA Synthesis	ssDNA from pTZ/pBluescript
Electrophoresis	Glyoxal gels
Hybridization	Hybridization
	Northerns
	Nitrocellulose Filter Hybridization
	Southerns
Labeling	End-labeling DNA fragments
	End-labeling RNA fragments
	5' End-labeling Alkaline Hydrolyzed rRNA
	Preparing 5' end-labeled alkaline hydrolyzed RNA
	Nick translation
	32P-pCp 3' End-labeling RNA
Others	DMS-treating DNA
	Figure 8 (C) test
	Filter-binding assay
	Poly(A) Assay Using ³H-Poly(U)
	RNA polymerase assay
Plasmid Isolation	Mini Cleard Lysate
	CsCl/Et Br plasmid isolation procedure
	Giant plasmid mini-prep
Protein Assay	Micro-protein assay
RNA Analysis	Primer extension analysis of RNA 5' ends
RNA Isolation	Isolation of total RNA using CsCl/EtBr gradients
	Eubacterial RNA isolation
	Precipitation of high MW RNAs by NaCl
	Sucrose Gradient for RNA
	RNase P RNA prep
	Single Step Prokaryotic RNA Isolation
RNA Synthesis	T7 run-off transcription
Screening	Colony lifts
Screening	Spot blots
Sequencing	ds-cDNA Sequencing
	Exo III sequencing deletions
	rRNA sequencing using reverse transcriptase
	Sequencing gels
cDNA Synthesis	³²P-cDNA synthesis
cDNA Synthesis	ds-cDNA synthesis
mRNA Isolation	Oligo (dT) Cellulose Batch Chromatography
	Oligo(dT)-cellulose
	Poly(U) sepharose
	Sucrose Gradient Sizing of Poly (A)⁺ RNA Using ³H-Poly(U)